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              首页 >> 产品 >> 标记抗体抗原 >> HRP标记 >> Phospho-AKT(Ser473) Antibody, HRP Conjugates
              Phospho-AKT(Ser473) Antibody, HRP Conjugates
              产品编号: ICP0455
              • 100.00μg
                ¥4500.00
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              详细介绍

              Phospho-AKT (Ser473) Antibody, HRP

              Catalog#

              Product Description

              ICP0455

              PKB alpha (AKT1) is a member of the PKB family, which is responsive to cell activation. The anti-PKB alpha antibodies were raised with a peptide based on the sequence of the human PKB C-terminal end and affinity purified with the same peptide on agarose. The affinity purified antibodies are useful for detection and isolation of PKB alpha from cell lysates.

              A, B : Western blot analysis of FAB treated cell lysate with anti-pPKB (Ser473). ICP0453 and a monoclonal anti-pPKB (Ser473) from other supplier.
              C: 5 ng/mL of FAB (anti-receptor) treatment with 10s                       D: Control - lymphocytes with no treatment
              IHC of Human Breast Cancer
              Species
              Rabbit
              Formulation
              250 μg/mL in PBS, 50% glycerol
              Immunogen
              Human PKB C-terminal peptide that is based on human PKB-KLH conjugates
              Purification
              The antibody was affinity purified with PKB phospho-peptide on agarose and affinity adsorption with non-phospho-peptide of the same sequence.
              Specificity
              Recognizes phosphorylated PKB on serine 473. There are no cross reactions with the non-phosphorylated PKB.
              Applications
              ELISA (0.25 μg/mL); WB (1 μg/mL); IP (10 μg/2 mg crude protein)
              Scientific Description
              AKT is also known as protein kinase B (PKB). It is an important molecule in mammalian cellular signalling. It inhibits apoptotic processes and is involved in cellular survival pathways. It also induces protein synthesis pathways.
              Storage & Stability
              Product is stable for several weeks at 4°C. For extended storage,  store product at -20°C.  Expiration date is six months from date of shipping if stored properly.
              Product Specific References
              1. J. Clin. Invest. 2003. 112: 197-208. doi:10.1172/JCI16885


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